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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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The effect of a nitric oxide donor on the synthesis of cGMP in Hymenolepis diminuta: a radiometric study.

The formation of cGMP in homogenates of the adult rat-tapeworm Hymenolepis diminuta was followed with a radiometric assay during 3 h after stimulation with the nitric oxide donor sodium nitroprusside (SNP) and in the presence of isobutylmethylxanthine (IBMX). The level of cGMP was stable in worms incubated with IBMX during the first hour. After 3 h of incubation, the level of cGMP had declined by 27%. Addition of SNP stimulated the formation of cGMP during the first hour of incubation. After 3 h of incubation, a two-fold decline in cGMP formation was observed. The rate of nitric oxide (NO) release by the worm was determined by a spectrophotometric assay for the accumulation of nitrites and nitrates, the stable degradation products of NO, using the Griess reaction. The results are discussed from the perspective of the current concept on the role of the nitrergic mechanisms in the flatworm nervous system.[1]

References

  1. The effect of a nitric oxide donor on the synthesis of cGMP in Hymenolepis diminuta: a radiometric study. Onufriev, M.V., Gulyaeva, N.V., Terenina, N.B., Tolstenkov, O.O., Gustafsson, M.K. Parasitol. Res. (2005) [Pubmed]
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