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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Identification of cholesterol-bound aldehydes in copper-oxidized low density lipoprotein.

Lipid-soluble cholesteryl ester core aldehydes (aldehydes still bound to the cholesterol ring) were identified among the products of copper-catalyzed peroxidation of human low density lipoprotein (LDL). The LDL was exposed to oxygenated buffer and 5 microM CuSO4 for 24 h. The core aldehydes were isolated as the dinitrophenylhydrazones, and were identified by reverse-phase HPLC with mass spectrometry. The major components were the C4- C10 oxoalkanoyl esters of cholesterol and 7-ketocholesterol, and accounted for 1-2% of the cholesteryl linoleate and arachidonate consumed.[1]

References

  1. Identification of cholesterol-bound aldehydes in copper-oxidized low density lipoprotein. Kamido, H., Kuksis, A., Marai, L., Myher, J.J. FEBS Lett. (1992) [Pubmed]
 
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