Enhanced glycerol production in Shochu yeast by heat-shock treatment is due to prolonged transcription of GPD1.
Enhancement of glycerol production in Shochu yeast, which was induced by heat-shock treatment, was studied. Although heat-shock treatment (45 degrees C, 1 h) caused a transient delay in cell growth, the amount of glycerol produced by heat-shock-treated cells was 20% higher than that by control cells. During the glycerol-production phase, the NAD+-dependent glycerol-3-phosphate dehydrogenase (GPDH) activity of heat-shock-treated cells was much higher than that of control cells, suggesting that a higher GPDH activity enhances glycerol production. The level of NAD+-dependent glycerol dehydrogenase (GDH) activity was almost the same between heat-shock-treated cells and control cells. The results of Northern blot analysis of GPD genes ( GPD1 and GPD2) encoding the GPDH enzyme showed that the transcription of GPD genes was not affected by heat-shock treatment but the period of intensive transcription of GPD1 was prolonged.[1]References
- Enhanced glycerol production in Shochu yeast by heat-shock treatment is due to prolonged transcription of GPD1. Kajiwara, Y., Ogawa, K., Takashita, H., Omori, T. J. Biosci. Bioeng. (2000) [Pubmed]
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