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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Accumulation of L-cystathionine by an Escherichia coli mutant deficient in cystathionine beta-lyase.

An Escherichia coli mutant deficient in cystathionine beta-lyase was found to accumulate a substance detectable by ninhydrin reaction and chloride platinic acid reaction in its cells (rarely in the culture supernatant) when cultured with a limited amount (50-200 microg/ml) of L-methionine to support the growth. The product was released by freezing treatment and isolated by ion-exchange chromatography (cation exchange resin: Daiaion SK1B). It was identified as L-cystathionine by liquid chromatography-mass spectrometry, 13C- and 1H-nuclear magnetic resonance analyses and high-performance liquid chromatography (as its 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate derivative).[1]

References

  1. Accumulation of L-cystathionine by an Escherichia coli mutant deficient in cystathionine beta-lyase. Nishi, T., Tanaka, K., Tanaka, Y., Araki, K., Furihata, K., Onodera, M., Toda, K. J. Biosci. Bioeng. (2002) [Pubmed]
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