Interaction of bHLH-PAS proteins involved in juvenile hormone reception in Drosophila.
The Methoprene-tolerant ( Met) bHLH-PAS gene is involved in juvenile hormone ( JH) action in Drosophila melanogaster as a likely component of a JH receptor. We expressed Met in Drosophila S2 cells and explored for MET partners using pull-down assays. MET-MET interaction was found to occur. The germ-cell expressed ( gce) gene is another D. melanogaster bHLH-PAS gene with high homology to Met, and GCE formed heterodimers with MET. In the presence of JH or either of two JH agonists, MET-MET and MET-GCE formation was drastically reduced. Interaction between GCE and MET having N- or C-terminus truncations, bHLH or PAS-A domain deletions, or a point mutation in the PAS-B domain failed to occur. However, JH-dependent interaction occurred between GCE and MET having point mutations in bHLH or PAS-A. During development, changes in JH titer may alter partner binding by MET and result in different gene expression patterns.[1]References
- Interaction of bHLH-PAS proteins involved in juvenile hormone reception in Drosophila. Godlewski, J., Wang, S., Wilson, T.G. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
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