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Gene Review

Met  -  Methoprene-tolerant

Drosophila melanogaster

Synonyms: CG1705, DmMet, Dmel\CG1705, MET, Mett, ...
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Disease relevance of Rst(1)JH

  • A Met(+) cDNA was expressed in Escherichia coli, and polyclonal antibody was prepared against the purified protein [1].

High impact information on Rst(1)JH

  • Transformation of Met flies with a Met+ cDNA resulted in susceptibility to methoprene, indicating that the cDNA encodes a functional Met+ protein [2].
  • Previous results have demonstrated a mechanism of resistance involving an intracellular JH binding protein that has reduced ligand affinity in Met flies [3].
  • Although Met27 homozygotes have reduced oogenesis, they are viable, demonstrating that Met is not a vital gene [3].
  • We cloned the Met+ gene by transposable P-element tagging and found reduced transcript level in several mutant alleles, showing that underproduction of the normal gene product can lead to insecticide resistance [2].
  • Male accessory gland cytosol from wild-type flies was found to contain a single binder with a dissociation constant (KD) of 6.7 nM for JH III; a binder in similar preparations from the methoprene-tolerant (Met) mutant had a KD value 6-fold higher [4].

Biological context of Rst(1)JH

  • Two of the alleles were shown to have complete P-elements inserted in similar, but not identical, locations in the predicted cytogenetic region where Met is located [5].
  • This determination was made by comparing the methoprene-resistance phenotype produced by representative Met alleles with that produced by a chromosome carrying a deficiency that deletes the Met gene [6].
  • P-element insertional alleles of Met were generated, and genomic libraries were constructed from two of these alleles [5].
  • Several alleles of Met have been recovered from methoprene selection screens after mutagenesis with ethyl methanesulfonate, X-rays, or transposable genetic elements [6].
  • Furthermore, the genetic covariance between developmental time and early fecundity attributed to alleles of Met was negative, indicating consistent pleiotropic effects among alleles on these traits [7].

Anatomical context of Rst(1)JH


Analytical, diagnostic and therapeutic context of Rst(1)JH

  • The molecular cloning of Met will be a step toward understanding this gene and possibly identifying a preadult role(s) for JH [5].


  1. Intracellular localization and tissue specificity of the Methoprene-tolerant (Met) gene product in Drosophila melanogaster. Pursley, S., Ashok, M., Wilson, T.G. Insect Biochem. Mol. Biol. (2000) [Pubmed]
  2. Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators. Ashok, M., Turner, C., Wilson, T.G. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  3. Insecticide resistance resulting from an absence of target-site gene product. Wilson, T.G., Ashok, M. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  4. Evidence for a juvenile hormone receptor involved in protein synthesis in Drosophila melanogaster. Shemshedini, L., Lanoue, M., Wilson, T.G. J. Biol. Chem. (1990) [Pubmed]
  5. Molecular analysis of the Methoprene-tolerant gene region of Drosophila melanogaster. Turner, C., Wilson, T.G. Arch. Insect Biochem. Physiol. (1995) [Pubmed]
  6. Genetic evidence that mutants of the methoprene-tolerant gene of Drosophila melanogaster are null mutants. Wilson, T.G. Arch. Insect Biochem. Physiol. (1996) [Pubmed]
  7. Pleiotropic effects of methoprene-tolerant (Met), a gene involved in juvenile hormone metabolism, on life history traits in Drosophila melanogaster. Flatt, T., Kawecki, T.J. Genetica (2004) [Pubmed]
  8. A Drosophila melanogaster mutant resistant to a chemical analog of juvenile hormone. Wilson, T.G., Fabian, J. Dev. Biol. (1986) [Pubmed]
  9. Interaction of bHLH-PAS proteins involved in juvenile hormone reception in Drosophila. Godlewski, J., Wang, S., Wilson, T.G. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
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