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Size prediction of recombinant human growth hormone nanoparticles produced by supercritical fluid precipitation.

Solution-enhanced dispersion by supercritical fluids (SEDS) was applied to produce nano-sized recombinant human growth hormone ( hGH) particles. Ethanol was used to help the supercritical carbon dioxide to extract water from the aqueous protein solution. Various sizes of hGH nanoparticles were successfully prepared with a narrow particle size distribution from aqueous ethanol solution without using any additive. The theoretical particle sizes were deduced from the calculated droplet sizes based on a modified Jasuja's equation. The calculated mean particle sizes and the experimentally obtained ones were compared and the results showed an excellent correlation coefficient (R (2)) of 0.995. Figure Distribution of hGH Nano-particles.[1]

References

  1. Size prediction of recombinant human growth hormone nanoparticles produced by supercritical fluid precipitation. Pyo, D., Lim, C., Cho, D., Oh, D. Analytical and bioanalytical chemistry (2007) [Pubmed]
 
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