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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX.

For investigating the feasibility of using reactive dyes as an immunoassay marker, a dichlorine triazine dye, Procion Blue MX-7RX, was employed to stain the antibody against human serum albumin (anti-HSA). With the color intensity revealed in the immunochromatographic test strip as the objective variables, the optimal dyeing conditions were found as follows: pH 11.4, temperature 35.7 degrees C, molar ratio 188 ( mol dye/ mol antibody), and reaction time 45.6min. The dyed-anti-HSA revealed a maximal color intensity of 8738 without apparent loss of antigen binding affinity.[1]

References

  1. Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX. Wang, S.J., Lin, H.M., Wang, X.R., Hsiung, K.P., Liu, Y.C. Anal. Biochem. (2007) [Pubmed]
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