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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Determination of furosine in biomedical samples employing an improved hydrolysis and high-performance liquid chromatographic technique.

Traditionally, the most sensitive and specific determination of non-enzymatic protein glycation has involved an 18-24-h acid hydrolysis in order to generate the compound furosine, which has been detected employing reversed-phase h.p.l.c. In this study, we have reported that significant quantities of furosine can be generated with much shorter hydrolysis times employing a 90-min vapor-phase acid hydrolysis procedure. The furosine generated by vapor-phase hydrolysis is then quantitated by pulsed amperometric detection using anion-exchange high-performance liquid chromatography. Employing this method, we were able to show that furosine generated from acid hydrolysis of purified hepatic membranes in a diabetic and non-diabetic animal model agreed with traditional methods assessing total glycated protein (i.e., boronate affinity methods).[1]

References

  1. Determination of furosine in biomedical samples employing an improved hydrolysis and high-performance liquid chromatographic technique. Cefalu, W.T., Bell-Farrow, A., Wang, Z.Q., Ralapati, S. Carbohydr. Res. (1991) [Pubmed]
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