Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Watanabe, Y. et al.

Yasuo Watanabe, Hidenori Akita, Yuka Higuchi, Rie Tsujimatsu, Tsuyoshi Kaneta, Youichi Tamai,

Heterologous expression of Na+/H+ antiporter gene (CvNHA1) from salt-tolerant yeast Candida versatilis in Saccharomyces cerevisiae Na+-transporter deficient mutants.

A Na(+)/H(+) antiporter gene (CvNHA1) was cloned from the salt-tolerant yeast Candida versatilis. CvNHA1 encodes an antiporter with a typical yeast plasma membrane Na(+)/H(+) antiporter structure. Transcription of CvNHA1 in C. versatilis cells was dependent on the salinity of the culture. When CvNHA1 was expressed in salt-sensitive Saccharomyces cerevisiae cells, increased salt-tolerance was observed, indicating that Cvnha1p possesses an Na(+)/H(+) antiporter function, because the increased salt-tolerance was dependent on the extracellular pH. It appears that Cvnha1p mediates only the transport of Na(+). In an S. cerevisiae transformant harboring a CvNHA1-EGFP fusion plasmid in which the greater part of the C-terminal hydrophilic region of Cvnha1p was deleted by fusion with enhanced green fluorescent protein (EGFP), the Cvnha1-EGFP fusion protein was localized mainly in the plasma membrane, and the NaCl-tolerance of this transformant was greater than that of a strain harboring the entire CvNHA1 gene.[1]

References

Heterologous expression of Na+/H+ antiporter gene (CvNHA1) from salt-tolerant yeast Candida versatilis in Saccharomyces cerevisiae Na+-transporter deficient mutants. Watanabe, Y., Akita, H., Higuchi, Y., Tsujimatsu, R., Kaneta, T., Tamai, Y. Biosci. Biotechnol. Biochem. (2008) [Pubmed]

Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.