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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

A role for asparaginyl-tRNA in the regulation of asparagine synthetase in a mammalian cell line.

The expression of asparagine synthetase activity [L-aspartate:ammonia ligase (AMP-forming), EC] in cultured Chinese hamster ovary (CHO) cells is regulated by asparagine. After transfer of CHO cells from an asparagine-supplemented medium to a medium lacking asparagine, activity increases 1.5- to 2-fold. If asparagine is added back to the medium, activity returns to control levels. To test the possible involvement of Asn-tRNAAsn in regulating the levels of asparagine synthetase, we have examined the levels of asparagine synthetase in a mutant of CHO cells containing a temperature-sensitive asparaginyl-tRNA synthetase [L-asparagine:tRNA ligase (AMP-forming), EC]. Under conditions of limited asparaginyl-tRNA synthetase activity in the mutant, there is a 2- to 3-fold increase in the level of asparagine synthetase activity. Under identical conditions, there is no change in asparagine synthetase activity in the wild type. This correlation between asparaginyl-tRNA synthetase activity and asparagine synthetase levels may be a consequence of a direct role of tRNAAsn in the regulation of the in vivo expression of the asparagine synthetase structural gene.[1]


  1. A role for asparaginyl-tRNA in the regulation of asparagine synthetase in a mammalian cell line. Arfin, S.M., Simpson, D.R., Chiang, C.S., Andrulis, I.L., Hatfield, G.W. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
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