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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

CHO Cells

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Disease relevance of CHO Cells


Psychiatry related information on CHO Cells

  • To test these possibilities, we have constructed stably transfected lines of CHO cells that express both wild-type mouse PrP and mouse PrP carrying an insertional mutation that is homologous to one associated with familial Creutzfeldt-Jakob disease [6].

High impact information on CHO Cells

  • A secreted fragment of the extracellular portion of human CD8 alpha has been expressed in CHO cells, and a deglycosylated and proteolyzed form of this fragment has been crystallized [7].
  • Transfection of the human alpha 5 and beta 1 cDNAs into transformed Chinese hamster ovary (CHO) cells followed by methotrexate-induced amplification yielded clonal cell lines overexpression this fibronectin receptor [8].
  • We have used two complementary two-dimensional gel electrophoretic methods to localize replication inititation sites and to determine replication fork direction in the amplified 240 kb dihydrofolate reductase domain of the methotrexate-resistant CHO cell line CHOC 400 [9].
  • This construct was transfected into CHO cells, and a cell line (CHO/TNF-20) that secretes TNF/cachectin was isolated [10].
  • CHO cells transfected with a TGF-alpha expression vector secrete high levels of TGF-alpha; a mixture of species of about 18 kd is secreted in addition to the 50 amino acid form [11].

Chemical compound and disease context of CHO Cells


Biological context of CHO Cells

  • This glycosylation occurs when G protein is transported during mixed incubations from the "donor" compartment in Golgi from VSV-infected CHO clone 15B cells (missing a key Golgi GlcNAc transferase) to the next, successive "acceptor" compartment (containing the GlcNAc transferase) in Golgi from wild-type CHO cells [16].
  • DNA synthesis is strongly activated by carbachol in those brain-derived cell lines and transfected CHO cells that express mAChR subtypes which activate PI hydrolysis efficiently, and poorly activated in cells expressing mAChR subtypes which only weakly activate PI hydrolysis [17].
  • An analysis of cycling cell populations (exponentially growing CHO cultures) and noncycling CHO cells arrested in the G1 phase by growth in isoleucine-free medium demonstrated the potential of the technique [18].
  • A CHO cell line with a single copy of the DHFR locus on chromosome Z2 was used to analyze the structure of the amplification target and products subsequent to the initial amplification event [19].
  • To elucidate the requirements for IRE1alpha and ATF6 for signaling the mammalian UPR, we identified a UPR reporter gene that was defective for induction in IRE1alpha-null mouse embryonic fibroblasts and S2P-deficient Chinese hamster ovary (CHO) cells [20].

Anatomical context of CHO Cells


Associations of CHO Cells with chemical compounds

  • We discuss the relevance of these findings to the mechanisms of glycoprotein biosynthesis in mammalian cells and to the biochemical bases of lectin resistance in CHO cells [26].
  • Regulatory mutations in CHO cells induce expression of the mouse embryonic antigen SSEA-1 [27].
  • The sensitivity to other lectins of these cells and of CHO cells resistant to concanavalin A (ConA) has been determined, and their activity of UDP-N-acetyl-glucosamine glycoprotein N-acetyl-glucosaminyltransferase (GlcNAc-T) has been measured [28].
  • Clones of CHO cells stably resistant to colcemid have been isolated in the presence of the nonionic detergent Tween 80 after mutagen treatment [29].
  • We report here that in Chinese hamster ovary (CHO) cells transfected with the D2 receptor complementary DNA, D2 agonists potently enhanced arachidonic acid release, provided that such release has been initiated by stimulating constitutive purinergic receptors or by increasing intracellular Ca2+ [30].

Gene context of CHO Cells

  • Fork slowing is reduced or absent in irs1SF CHO cells and XRCC3(-/-) chicken DT40 cells, indicating that fork slowing is an active process that requires the homologous recombination protein XRCC3 [31].
  • No detectable binding was observed to CD59-transfected CHO cells despite a report suggesting that CD59 may bind to the human CD2 adhesion domain [22].
  • Addition to primary cultures of the intact bivalent anti-CD28 mAb 9.3, or B7/BB1+ transfected CHO cells or exogenous IL-2, abrogated induction of hyporesponsiveness by CTLA4Ig [32].
  • The function of CD28-B7 interactions during T cell activation was investigated with soluble fusion proteins and with B7-transfected CHO cells [33].
  • This conclusion is based on the findings that IP-10 binding to cells is: (a) inhibited by heparin and heparan sulfate; (b) sensitive to a 1 M NaCl wash; (c) eliminated by treatment with heparinase and trypsin; and (d) absent on mutant CHO cells that do not express cell surface HSPG [34].

Analytical, diagnostic and therapeutic context of CHO Cells


  1. Identification of a human cDNA encoding a functional high affinity lipoxin A4 receptor. Fiore, S., Maddox, J.F., Perez, H.D., Serhan, C.N. J. Exp. Med. (1994) [Pubmed]
  2. Interaction of Ipa proteins of Shigella flexneri with alpha5beta1 integrin promotes entry of the bacteria into mammalian cells. Watarai, M., Funato, S., Sasakawa, C. J. Exp. Med. (1996) [Pubmed]
  3. Efficient expression of rat brain type IIA Na+ channel alpha subunits in a somatic cell line. West, J.W., Scheuer, T., Maechler, L., Catterall, W.A. Neuron (1992) [Pubmed]
  4. Complementation of sensitivity to alkylating agents in Escherichia coli and Chinese hamster ovary cells by expression of a cloned bacterial DNA repair gene. Kataoka, H., Hall, J., Karran, P. EMBO J. (1986) [Pubmed]
  5. Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans. Shieh, M.T., WuDunn, D., Montgomery, R.I., Esko, J.D., Spear, P.G. J. Cell Biol. (1992) [Pubmed]
  6. A wild-type prion protein does not acquire properties of the scrapie isoform when coexpressed with a mutant prion protein in cultured cells. Lehmann, S., Daude, N., Harris, D.A. Brain Res. Mol. Brain Res. (1997) [Pubmed]
  7. Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution. Leahy, D.J., Axel, R., Hendrickson, W.A. Cell (1992) [Pubmed]
  8. Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells. Giancotti, F.G., Ruoslahti, E. Cell (1990) [Pubmed]
  9. Replication initiates in a broad zone in the amplified CHO dihydrofolate reductase domain. Vaughn, J.P., Dijkwel, P.A., Hamlin, J.L. Cell (1990) [Pubmed]
  10. Tumors secreting human TNF/cachectin induce cachexia in mice. Oliff, A., Defeo-Jones, D., Boyer, M., Martinez, D., Kiefer, D., Vuocolo, G., Wolfe, A., Socher, S.H. Cell (1987) [Pubmed]
  11. Different transforming growth factor-alpha species are derived from a glycosylated and palmitoylated transmembrane precursor. Bringman, T.S., Lindquist, P.B., Derynck, R. Cell (1987) [Pubmed]
  12. Transport of newly synthesized vesicular stomatitis viral glycoprotein to purified Golgi membranes. Rothman, J.E., Fries, E. J. Cell Biol. (1981) [Pubmed]
  13. GLUT-4 NH2 terminus contains a phenylalanine-based targeting motif that regulates intracellular sequestration. Piper, R.C., Tai, C., Kulesza, P., Pang, S., Warnock, D., Baenziger, J., Slot, J.W., Geuze, H.J., Puri, C., James, D.E. J. Cell Biol. (1993) [Pubmed]
  14. Enhancement of ricin cytotoxicity in Chinese hamster ovary cells by depletion of intracellular K+: evidence for an Na+/H+ exchange system in Chinese hamster ovary cells. Ghosh, P.C., Wellner, R.B., Cragoe, E.J., Wu, H.C. J. Cell Biol. (1985) [Pubmed]
  15. Enhancement of cytotoxicities of ricin and Pseudomonas toxin in Chinese hamster ovary cells by nigericin. Ray, B., Wu, H.C. Mol. Cell. Biol. (1981) [Pubmed]
  16. Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine. Balch, W.E., Dunphy, W.G., Braell, W.A., Rothman, J.E. Cell (1984) [Pubmed]
  17. Acetylcholine analogue stimulates DNA synthesis in brain-derived cells via specific muscarinic receptor subtypes. Ashkenazi, A., Ramachandran, J., Capon, D.J. Nature (1989) [Pubmed]
  18. Correlated measurements of DNA, RNA, and protein in individual cells by flow cytometry. Crissman, H.A., Darzynkiewicz, Z., Tobey, R.A., Steinkamp, J.A. Science (1985) [Pubmed]
  19. A central role for chromosome breakage in gene amplification, deletion formation, and amplicon integration. Windle, B., Draper, B.W., Yin, Y.X., O'Gorman, S., Wahl, G.M. Genes Dev. (1991) [Pubmed]
  20. IRE1-mediated unconventional mRNA splicing and S2P-mediated ATF6 cleavage merge to regulate XBP1 in signaling the unfolded protein response. Lee, K., Tirasophon, W., Shen, X., Michalak, M., Prywes, R., Okada, T., Yoshida, H., Mori, K., Kaufman, R.J. Genes Dev. (2002) [Pubmed]
  21. Morphological changes in cultured mammalian cells: prevention by the calcium ionophore A23187. Henneberry, R.C., Fishman, P.H., Freese, E. Cell (1975) [Pubmed]
  22. A soluble multimeric recombinant CD2 protein identifies CD48 as a low affinity ligand for human CD2: divergence of CD2 ligands during the evolution of humans and mice. Arulanandam, A.R., Moingeon, P., Concino, M.F., Recny, M.A., Kato, K., Yagita, H., Koyasu, S., Reinherz, E.L. J. Exp. Med. (1993) [Pubmed]
  23. Human Mig chemokine: biochemical and functional characterization. Liao, F., Rabin, R.L., Yannelli, J.R., Koniaris, L.G., Vanguri, P., Farber, J.M. J. Exp. Med. (1995) [Pubmed]
  24. Role of platelet-activating factor in Chinese hamster ovary cell responses to cholera toxin. Thielman, N.M., Marcinkiewicz, M., Sarosiek, J., Fang, G.D., Guerrant, R.L. J. Clin. Invest. (1997) [Pubmed]
  25. Comparative analysis of natural killer cell and macrophage recognition of concanavalin A-resistant Chinese hamster ovary cells: role of membrane oligosaccharides. Pohajdak, B., Lee, K.C., Sugawara, I., Miller, V., Wright, J.A., Greenberg, A.H. J. Natl. Cancer Inst. (1986) [Pubmed]
  26. Specific changes in the oligosaccharide moieties of VSV grown in different lectin-resistnat CHO cells. Robertson, M.A., Etchison, J.R., Robertson, J.S., Summers, D.F., Stanley, P. Cell (1978) [Pubmed]
  27. Regulatory mutations in CHO cells induce expression of the mouse embryonic antigen SSEA-1. Campbell, C., Stanley, P. Cell (1983) [Pubmed]
  28. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cell. Stanley, P., Caillibot, V., Siminovitch, L. Cell (1975) [Pubmed]
  29. Mutants of Chinese hamster ovary (CHO) cells with altered colcemid-binding affinity. Ling, V., Aubin, J.E., Chase, A., Sarangi, F. Cell (1979) [Pubmed]
  30. Dopamine activation of the arachidonic acid cascade as a basis for D1/D2 receptor synergism. Piomelli, D., Pilon, C., Giros, B., Sokoloff, P., Martres, M.P., Schwartz, J.C. Nature (1991) [Pubmed]
  31. XRCC3 and Rad51 modulate replication fork progression on damaged vertebrate chromosomes. Henry-Mowatt, J., Jackson, D., Masson, J.Y., Johnson, P.A., Clements, P.M., Benson, F.E., Thompson, L.H., Takeda, S., West, S.C., Caldecott, K.W. Mol. Cell (2003) [Pubmed]
  32. Induction of alloantigen-specific hyporesponsiveness in human T lymphocytes by blocking interaction of CD28 with its natural ligand B7/BB1. Tan, P., Anasetti, C., Hansen, J.A., Melrose, J., Brunvand, M., Bradshaw, J., Ledbetter, J.A., Linsley, P.S. J. Exp. Med. (1993) [Pubmed]
  33. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. Linsley, P.S., Brady, W., Grosmaire, L., Aruffo, A., Damle, N.K., Ledbetter, J.A. J. Exp. Med. (1991) [Pubmed]
  34. The IP-10 chemokine binds to a specific cell surface heparan sulfate site shared with platelet factor 4 and inhibits endothelial cell proliferation. Luster, A.D., Greenberg, S.M., Leder, P. J. Exp. Med. (1995) [Pubmed]
  35. An alteration in the phosphorylation of vimentin-type intermediate filaments is associated with mitosis in cultured mammalian cells. Evans, R.M., Fink, L.M. Cell (1982) [Pubmed]
  36. Gene targeting in normal and amplified cell lines. Zheng, H., Wilson, J.H. Nature (1990) [Pubmed]
  37. The secretory proprotein convertases furin, PC5, and PC7 activate VEGF-C to induce tumorigenesis. Siegfried, G., Basak, A., Cromlish, J.A., Benjannet, S., Marcinkiewicz, J., Chrétien, M., Seidah, N.G., Khatib, A.M. J. Clin. Invest. (2003) [Pubmed]
  38. Localization of the catalytic subunit of cyclic AMP-dependent. Protein kinase in cultured cells using a specific antibody. Murtaugh, M.P., Steiner, A.L., Davies, P.J. J. Cell Biol. (1982) [Pubmed]
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