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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

A general ampC active site oligonucleotide probe for gram-negative rods.

We have developed a 33 mer probe that hybridizes to the serine active site of the chromosomal ampC beta-lactamase gene of Pseudomonas aeruginosa and the Enterobacteriaceae. We tested this probe against a variety of Enterobacteriaceae, and a series of 23 P. aeruginosa by dot-blots and selected Southern blots. This probe is an alternative or supplement to enzyme studies for characterizing the class of a Gram-negative rod's beta-lactamase and is a useful tool for studies of Pseudomonas beta-lactamase regulation.[1]

References

  1. A general ampC active site oligonucleotide probe for gram-negative rods. Willard, K.E., Moody, J.A., Peterson, L.R. Mol. Cell. Probes (1991) [Pubmed]
 
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