The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Actively synthesizing beta-cells secrete preferentially after glucose stimulation.

To establish whether the heterogeneous secretion of glucose-stimulated beta-cells correlates with a different biosynthetic activity, we have studied the secretion and biosynthesis of the very same beta-cells by combining a hemolytic plaque assay with autoradiography. After a 10-min incubation in 2.8 mM glucose, 52 +/- 2% of dispersed rat beta-cells incorporated [3H] leucine into newly synthesized proteins, as revealed by autoradiographic labeling. When the incubation was performed in 16.7 mM glucose, larger (P less than 0.02) proportions (92 +/- 4%) of plaque-forming, i.e. insulin-secreting, and nonplaque-forming beta-cells (74 +/- 4%) were autoradiographically labeled. Labeled and unlabeled beta-cells were stimulated to secrete insulin during a 30-min incubation in 16.7 mM glucose, as revealed by the larger (P less than 0.001) formation of hemolytic plaques. Under these conditions, autoradiographically labeled beta-cells were recruited preferentially (P less than 0.01) and secreted more (P less than 0.04) than unlabeled beta-cells. Analogous observations were made with beta-cell pairs. Under glucose stimulation, pairs comprising two autoradiographically labeled beta-cells secreted more (P less than 0.004) than pairs comprising one or no labeled beta-cells. The data indicate that under glucose stimulation, 1) secreting and nonsecreting beta-cells increase protein biosynthesis; 2) biosynthetically active and inactive beta-cells increase insulin secretion; 3) beta-cells synthesizing new proteins release insulin preferentially; and 4) contact decreases the biosynthetic and secretory heterogeneity of beta-cells.[1]


WikiGenes - Universities