Brain dynein (MAP1C) localizes on both anterogradely and retrogradely transported membranous organelles in vivo.
Brain dynein is a microtubule-activated ATPase considered to be a candidate to function as a molecular motor to transport membranous organelles retrogradely in the axon. To determine whether brain dynein really binds to retrogradely transported organelles in vivo and how it is transported to the nerve terminals, we studied the localization of brain dynein in axons after the ligation of peripheral nerves by light and electron microscopic immunocytochemistry using affinity-purified anti-brain dynein antibodies. Different classes of organelles preferentially accumulated at the regions proximal and distal to the ligated part. Interestingly, brain dynein accumulated both at the regions proximal and distal to the ligation sites and localized not only on retrogradely transported membranous organelles but also on anterogradely transported ones. This is the first evidence to show that brain dynein associates with retrogradely transported organelles in vivo and that brain dynein is transported to the nerve terminal by fast flow. This also suggests that there may be some mechanism that activates brain dynein only for retrograde transport.[1]References
- Brain dynein (MAP1C) localizes on both anterogradely and retrogradely transported membranous organelles in vivo. Hirokawa, N., Sato-Yoshitake, R., Yoshida, T., Kawashima, T. J. Cell Biol. (1990) [Pubmed]
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