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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Detection of several novel gamma-aminobutyric acid-containing compounds in human CSF.

gamma-Aminobutyric acid (GABA) concentrations in human CSF are known to increase significantly after hydrolysis; however, the source of this increase has been unknown. Using either ion-exchange or reverse-phase chromatography coupled with on-line alkaline hydrolysis, we have shown 2-pyrrolidinone, the lactam of GABA, to be present in insufficient quantity to account for this increase. Subsequent experiments involving fraction collection of column eluents followed by acid hydrolysis and rechromatography demonstrated the presence of several previously undetected GABA-containing compounds.[1]

References

  1. Detection of several novel gamma-aminobutyric acid-containing compounds in human CSF. Miller, J.M., Ferraro, T.N., Hare, T.A. J. Neurochem. (1990) [Pubmed]
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