Specificity of binding to four-way junctions in DNA by bacteriophage T7 endonuclease I.
T7 endonuclease I binds specifically to four-way junctions in duplex DNA and promotes their resolution into linear duplexes. Under conditions in which the nuclease activity is blocked by the absence of divalent cations, the enzyme forms a distinct protein-DNA complex with the junction, as detected by gel retardation and filter binding assays. The formation of this complex is structure-specific and contrasts with the short-lived binding complexes formed on linear duplex DNA. The binding complex between T7 endonuclease I and a synthetic Holliday junction analog has been probed with hydroxyl radicals. The results indicate that the nuclease binds all four strands about the junction point.[1]References
- Specificity of binding to four-way junctions in DNA by bacteriophage T7 endonuclease I. Parsons, C.A., West, S.C. Nucleic Acids Res. (1990) [Pubmed]
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