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Gene Review

T7p19  -  endonuclease I

Enterobacteria phage T7

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Disease relevance of T7p19

  • The phage T7 endonuclease gene was fused to the 3' end of the lac repressor gene [1].
  • When DNA expelled from previously cross-linked T7 is cleaved with restriction endonuclease (1 to 3 sites cleaved), analysis of the resulting fragments reveals no regions on T7 DNA that are excluded from cross-linking to the capsid [2].
  • Effect of DNA topology on Holliday junction resolution by Escherichia coli RuvC and bacteriophage T7 endonuclease I [3].

High impact information on T7p19

  • Thus, cleavage by T7 endonuclease I displays great structural specificity with an efficiency that can vary slightly according to the DNA sequence [4].
  • The binding complex between T7 endonuclease I and a synthetic Holliday junction analog has been probed with hydroxyl radicals [5].
  • Our data show that the form of the complex between endonuclease I and a DNA junction depends on the core of the junction and on interactions with the first six base-pairs of the arms containing the 5' ends of the continuous strands [6].
  • Restriction endonuclease kinetic analysis reveals that proflavine (8 micrograms/ml) completely blocks formation of the mature T7 DNA left end, but only partially blocks formation of the mature T7 DNA right end [7].
  • (1) Most degradation of intracellular DNA requires the presence of T7 gene 3 endonuclease and is independent of DNA packaging; rapidly sedimenting, branched DNA accumulates when both the gene 3 and gene 6 products are absent [8].

Biological context of T7p19

  • The various activities of gene 3 endonuclease are consistent with the known role of this enzyme in genetic recombination, in maturation and packaging of T7 DNA, and in degradation of host DNA, and suggest that the enzyme recognizes a specific structural feature in DNA [9].
  • Also, we examined the difference in the extent and temperature dependence of promoter unwinding in the two complexes, as probed by methylation of unpaired cytosines and cleavage by phage T7 endonuclease [10].
  • The variable positions of a branch-migrating cruciform junction in supercoiled plasmid DNA were mapped following cleavage of the DNA with bacteriophage T7 endonuclease I. T7 endonuclease I specifically cleaved, and thereby resolved, the Holliday junction existing at the base of the cruciform in the circular bacterial plasmid pSA1B.56A [11].
  • The recognition sequence 5'-CC(A/T)GG for EcoRII in the bacteriophage T7 genome is refractory to this restriction endonuclease, despite not bearing the specific (protective) methylation [12].

Associations of T7p19 with chemical compounds

  • T4 endonuclease VII showed a cleavage preference for the 3' side of thymine bases, whereas T7 endonuclease I preferentially cut the DNA between two pyrimidine residues [13].

Other interactions of T7p19


Analytical, diagnostic and therapeutic context of T7p19


  1. Biosynthesis of a repressor/nuclease hybrid protein. Panayotatos, N., Fontaine, A., Bãckman, S. J. Biol. Chem. (1989) [Pubmed]
  2. Conformation of DNA packaged in bacteriophage T7. Analysis by use of ultraviolet light-induced DNA-capsid cross-linking. Serwer, P., Hayes, S.J., Watson, R.H. J. Mol. Biol. (1992) [Pubmed]
  3. Effect of DNA topology on Holliday junction resolution by Escherichia coli RuvC and bacteriophage T7 endonuclease I. Zerbib, D., Colloms, S.D., Sherratt, D.J., West, S.C. J. Mol. Biol. (1997) [Pubmed]
  4. The site-specific cleavage of synthetic Holliday junction analogs and related branched DNA structures by bacteriophage T7 endonuclease I. Dickie, P., McFadden, G., Morgan, A.R. J. Biol. Chem. (1987) [Pubmed]
  5. Specificity of binding to four-way junctions in DNA by bacteriophage T7 endonuclease I. Parsons, C.A., West, S.C. Nucleic Acids Res. (1990) [Pubmed]
  6. Structural recognition between a four-way DNA junction and a resolving enzyme. Déclais, A.C., Liu, J., Freeman, A.D., Lilley, D.M. J. Mol. Biol. (2006) [Pubmed]
  7. Formation of the right before the left mature DNA end during packaging-cleavage of bacteriophage T7 DNA concatemers. Serwer, P., Watson, R.H., Hayes, S.J. J. Mol. Biol. (1992) [Pubmed]
  8. Role of gene 6 exonuclease in the replication and packaging of bacteriophage T7 DNA. Serwer, P., Watson, R.H., Son, M. J. Mol. Biol. (1990) [Pubmed]
  9. Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA. de Massy, B., Weisberg, R.A., Studier, F.W. J. Mol. Biol. (1987) [Pubmed]
  10. Comparison of the open complexes formed by RNA polymerase at the Escherichia coli lac UV5 promoter. Straney, D.C., Crothers, D.M. J. Mol. Biol. (1987) [Pubmed]
  11. Conformational isomerization of the Holliday junction associated with a cruciform during branch migration in supercoiled plasmid DNA. Dickie, P., Morgan, A.R., McFadden, G. J. Mol. Biol. (1988) [Pubmed]
  12. Cloning of the resistant EcoRII recognition site of phage T7 into an EcoRII-sensitive plasmid makes the site susceptible to the restriction enzyme. Krüger, D.H., Prösch, S., Reuter, M., Goebel, W. J. Basic Microbiol. (1990) [Pubmed]
  13. Cleavage specificity of bacteriophage T4 endonuclease VII and bacteriophage T7 endonuclease I on synthetic branch migratable Holliday junctions. Picksley, S.M., Parsons, C.A., Kemper, B., West, S.C. J. Mol. Biol. (1990) [Pubmed]
  14. Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro. Lee, D.D., Sadowski, P.D. J. Virol. (1982) [Pubmed]
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