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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Solubilization of receptor for pituitary adenylate cyclase activating polypeptide from bovine brain.

A receptor for pituitary adenylate cyclase activating polypeptide (PACAP) was solubilized from bovine brain membranes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Saturable binding experiments using [125I]PACAP27 revealed that the solubilized extract contained a single class of binding sites with a Kd of 200 pM and a Bmax of 0.9 pmol/mg protein. The Kd did not change significantly after the solubilization. Competitive binding experiments confirmed that the solubilized receptor retained a specificity for PACAP. Thus, the solubilization of the receptor in an active form was successfully achieved by the present procedure.[1]

References

  1. Solubilization of receptor for pituitary adenylate cyclase activating polypeptide from bovine brain. Masuda, Y., Ohtaki, T., Kitada, C., Tsuda, M., Arimura, A., Fujino, M. Biochem. Biophys. Res. Commun. (1990) [Pubmed]
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