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Kurosawa, N. et al.

Determination of streptomycin in serum by high-performance liquid chromatography.

A high-performance liquid chromatographic method was developed for monitoring the serum concentration of streptomycin. The method includes clean-up using a Sep-Pak C18 cartridge and quantitation using dihydrostreptomycin as an internal standard. Streptomycin and dihydrostreptomycin were separated by reversed-phase ion-pair chromatography on LiChrosorb RP-18 and detected by UV absorption (195 nm). The calibration graph of serum streptomycin concentration was linear over the range 5-50 micrograms/ml. Streptomycin was added to serum at the level of 20.0 micrograms/ml and its concentration was determined to be 18.9 micrograms/ml with a coefficient of variation of 2.07% (n = 5). The clinical application of this method was confirmed by comparison with fluorescence polarization immunoassay.[1]

References

Determination of streptomycin in serum by high-performance liquid chromatography. Kurosawa, N., Kuribayashi, S., Owada, E., Ito, K., Nioka, M., Arakawa, M., Fukuda, R. J. Chromatogr. (1985) [Pubmed]

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