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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Hydroxyindole-O-methyltransferase in Y-79 human retinoblastoma cells: effect of cell attachment.

Hydroxyindole-O-methyltransferase (HIOMT), the enzyme in the final step of melatonin synthesis, is present in the Y-79 human retinoblastoma cell line. Using electroblot immunolabellings, a single band corresponding to HIOMT was observed. Immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and quantification of enzyme activity all revealed dramatic increases in HIOMT in cells attached to substrate compared to cells in suspension culture.[1]

References

  1. Hydroxyindole-O-methyltransferase in Y-79 human retinoblastoma cells: effect of cell attachment. Kyritsis, A.P., Wiechmann, A.F., Bok, D., Chader, G.J. J. Neurochem. (1987) [Pubmed]
 
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