The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Fluorescent Antibody Technique

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Fluorescent Antibody Technique


Psychiatry related information on Fluorescent Antibody Technique


High impact information on Fluorescent Antibody Technique


Chemical compound and disease context of Fluorescent Antibody Technique


Biological context of Fluorescent Antibody Technique


Anatomical context of Fluorescent Antibody Technique


Associations of Fluorescent Antibody Technique with chemical compounds

  • Both acetone and formaldehyde fixation were used for the immunofluorescence tests [2].
  • To test this hypothesis, we determined the level of fetal hemoglobin in dead and living infants in three different laboratories by three methods: high-performance liquid chromatography, polyacrylamide-gel electrophoresis, and cell-based immunofluorescence assays for fetal hemoglobin-containing red cells (F cells) [27].
  • The sensitivity of the three stains in detecting P. carinii was 45 of 49 (92 percent) for immunofluorescence; 37 of 49 (76 percent) for Diff-Quik (a Giemsa-type stain); and 39 of 49 (80 percent) for toluidine blue O [28].
  • Tunicamycin had only a slight effect on the initial times and rates of CSP appearance on the cell surface; some apparent intracellular redistribution of CSP was detected by immunofluorescence [29].
  • Genes for the lamina lucida protein, kalinin/laminin 5, have been proposed as candidates for some forms of JEB, based on immunofluorescence analysis recognizing kalinin epitopes [30].

Gene context of Fluorescent Antibody Technique

  • Immunofluorescence localization studies using affinity-purified antibody directed against the YPT1 protein showed punctate staining of the cytoplasm of growing yeast cells and very intense staining of small buds, where membrane growth and secretion are most active [31].
  • We have cloned a gene encoding one of these proteins (NUP1) and have confirmed the localization of the NUP1 protein to the pore complex by immunofluorescence, using an epitope-tagged construct to differentiate it from other members of this family [32].
  • Immunofluorescence studies demonstrate that both LANP and ataxin-1 colocalize in nuclear matrix-associated subnuclear structures [33].
  • Coimmunoprecipitations and immunofluorescence studies revealed that GIT1 and huntingtin associate in mammalian cells under physiological conditions [34].
  • Immunofluorescence analysis revealed a myelomonocytoid immunophenotype (expression of CD13 and CD68, and lack of lymphoid markers) [35].

Analytical, diagnostic and therapeutic context of Fluorescent Antibody Technique


  1. Yersinia antigens in synovial-fluid cells from patients with reactive arthritis. Granfors, K., Jalkanen, S., von Essen, R., Lahesmaa-Rantala, R., Isomäki, O., Pekkola-Heino, K., Merilahti-Palo, R., Saario, R., Isomäki, H., Toivanen, A. N. Engl. J. Med. (1989) [Pubmed]
  2. Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product. Rohrschneider, L.R. Cell (1979) [Pubmed]
  3. Apoprotein A-I synthesis in normal intestinal mucosa and in Tangier disease. Glickman, R.M., Green, P.H., Lees, R.S., Tall, A. N. Engl. J. Med. (1978) [Pubmed]
  4. Retinoblastoma--origin from a primitive neuroectodermal cell? Kyritsis, A.P., Tsokos, M., Triche, T.J., Chader, G.J. Nature (1984) [Pubmed]
  5. Sequence of the envelope glycoprotein gene of type II human T lymphotropic virus. Sodroski, J., Patarca, R., Perkins, D., Briggs, D., Lee, T.H., Essex, M., Coligan, J., Wong-Staal, F., Gallo, R.C., Haseltine, W.A. Science (1984) [Pubmed]
  6. Progressive transformation of the cytoskeleton associated with normal aging and Alzheimer's disease. Vickers, J.C., Delacourte, A., Morrison, J.H. Brain Res. (1992) [Pubmed]
  7. Neutrophil elastase cleaves PML-RARalpha and is important for the development of acute promyelocytic leukemia in mice. Lane, A.A., Ley, T.J. Cell (2003) [Pubmed]
  8. Sublimiting concentration of TFIIH transcription/DNA repair factor causes TTD-A trichothiodystrophy disorder. Vermeulen, W., Bergmann, E., Auriol, J., Rademakers, S., Frit, P., Appeldoorn, E., Hoeijmakers, J.H., Egly, J.M. Nat. Genet. (2000) [Pubmed]
  9. The pallid gene encodes a novel, syntaxin 13-interacting protein involved in platelet storage pool deficiency. Huang, L., Kuo, Y.M., Gitschier, J. Nat. Genet. (1999) [Pubmed]
  10. Cse4p is a component of the core centromere of Saccharomyces cerevisiae. Meluh, P.B., Yang, P., Glowczewski, L., Koshland, D., Smith, M.M. Cell (1998) [Pubmed]
  11. Antibodies to butyrate-inducible antigens of Kaposi's sarcoma-associated herpesvirus in patients with HIV-1 infection. Miller, G., Rigsby, M.O., Heston, L., Grogan, E., Sun, R., Metroka, C., Levy, J.A., Gao, S.J., Chang, Y., Moore, P. N. Engl. J. Med. (1996) [Pubmed]
  12. Primary liver carcinoma and liver cirrhosis in atomic bomb survivors, Hiroshima and Nagasaki, 1961-75, with special reference to hepatitis B surface antigen. Asano, M., Kato, H., Yoshimoto, K., Seyama, S., Itakura, H., Hamada, T., Iijima, S. J. Natl. Cancer Inst. (1982) [Pubmed]
  13. Production of casein and the presence of estrogen receptors in human breast cancer. Pich, A., Bussolati, G., Di Carlo, F. J. Natl. Cancer Inst. (1977) [Pubmed]
  14. Quantitation of nitrotyrosine levels in lung sections of patients and animals with acute lung injury. Haddad, I.Y., Pataki, G., Hu, P., Galliani, C., Beckman, J.S., Matalon, S. J. Clin. Invest. (1994) [Pubmed]
  15. Proteinuria in passive Heymann nephritis is associated with lipid peroxidation and formation of adducts on type IV collagen. Neale, T.J., Ojha, P.P., Exner, M., Poczewski, H., Rüger, B., Witztum, J.L., Davis, P., Kerjaschki, D. J. Clin. Invest. (1994) [Pubmed]
  16. Selective modulation of PKC isozymes by inflammation in canine colonic circular muscle cells. Ali, I., Sarna, S.K. Gastroenterology (2002) [Pubmed]
  17. The metaphase scaffold is helically folded: sister chromatids have predominantly opposite helical handedness. Boy de la Tour, E., Laemmli, U.K. Cell (1988) [Pubmed]
  18. SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres. Palladino, F., Laroche, T., Gilson, E., Axelrod, A., Pillus, L., Gasser, S.M. Cell (1993) [Pubmed]
  19. The growth arrest-specific gene, gas1, is involved in growth suppression. Del Sal, G., Ruaro, M.E., Philipson, L., Schneider, C. Cell (1992) [Pubmed]
  20. Simultaneous flow cytometric analysis of human T cell activation antigen expression and DNA content. Cotner, T., Williams, J.M., Christenson, L., Shapiro, H.M., Strom, T.B., Strominger, J. J. Exp. Med. (1983) [Pubmed]
  21. Bcl-2-dependent oxidation of pyruvate dehydrogenase-E2, a primary biliary cirrhosis autoantigen, during apoptosis. Odin, J.A., Huebert, R.C., Casciola-Rosen, L., LaRusso, N.F., Rosen, A. J. Clin. Invest. (2001) [Pubmed]
  22. Characterization of cross-reacting antigens on the Epstein-Barr virus envelope and plasma membranes of producer cells. Thorley-Lawson, D.A. Cell (1979) [Pubmed]
  23. Mouse egg integrin alpha 6 beta 1 functions as a sperm receptor. Almeida, E.A., Huovila, A.P., Sutherland, A.E., Stephens, L.E., Calarco, P.G., Shaw, L.M., Mercurio, A.M., Sonnenberg, A., Primakoff, P., Myles, D.G., White, J.M. Cell (1995) [Pubmed]
  24. Disruption of the in vivo distribution of the intermediate filaments in fibroblasts through the microinjection of a specific monoclonal antibody. Lin, J.J., Feramisco, J.R. Cell (1981) [Pubmed]
  25. The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts. Singer, I.I. Cell (1979) [Pubmed]
  26. The synthesis and distribution of desmin and vimentin during myogenesis in vitro. Gard, D.L., Lazarides, E. Cell (1980) [Pubmed]
  27. Normal fetal hemoglobin levels in the sudden infant death syndrome. Zielke, H.R., Meny, R.G., O'Brien, M.J., Smialek, J.E., Kutlar, F., Huisman, T.H., Dover, G.J. N. Engl. J. Med. (1989) [Pubmed]
  28. Diagnosis of Pneumocystis carinii pneumonia: improved detection in sputum with use of monoclonal antibodies. Kovacs, J.A., Ng, V.L., Masur, H., Leoung, G., Hadley, W.K., Evans, G., Lane, H.C., Ognibene, F.P., Shelhamer, J., Parrillo, J.E. N. Engl. J. Med. (1988) [Pubmed]
  29. Role of carbohydrates in protein secretion and turnover: effects of tunicamycin on the major cell surface glycoprotein of chick embryo fibroblasts. Olden, K., Pratt, R.M., Yamada, K.M. Cell (1978) [Pubmed]
  30. Mutations in the gamma 2 chain gene (LAMC2) of kalinin/laminin 5 in the junctional forms of epidermolysis bullosa. Pulkkinen, L., Christiano, A.M., Airenne, T., Haakana, H., Tryggvason, K., Uitto, J. Nat. Genet. (1994) [Pubmed]
  31. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Segev, N., Mulholland, J., Botstein, D. Cell (1988) [Pubmed]
  32. The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Davis, L.I., Fink, G.R. Cell (1990) [Pubmed]
  33. The cerebellar leucine-rich acidic nuclear protein interacts with ataxin-1. Matilla, A., Koshy, B.T., Cummings, C.J., Isobe, T., Orr, H.T., Zoghbi, H.Y. Nature (1997) [Pubmed]
  34. A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease. Goehler, H., Lalowski, M., Stelzl, U., Waelter, S., Stroedicke, M., Worm, U., Droege, A., Lindenberg, K.S., Knoblich, M., Haenig, C., Herbst, M., Suopanki, J., Scherzinger, E., Abraham, C., Bauer, B., Hasenbank, R., Fritzsche, A., Ludewig, A.H., Büssow, K., Buessow, K., Coleman, S.H., Gutekunst, C.A., Landwehrmeyer, B.G., Lehrach, H., Wanker, E.E. Mol. Cell (2004) [Pubmed]
  35. Characterization of a novel Hodgkin cell line, HD-MyZ, with myelomonocytic features mimicking Hodgkin's disease in severe combined immunodeficient mice. Bargou, R.C., Mapara, M.Y., Zugck, C., Daniel, P.T., Pawlita, M., Döhner, H., Dörken, B. J. Exp. Med. (1993) [Pubmed]
  36. Mice lacking vimentin develop and reproduce without an obvious phenotype. Colucci-Guyon, E., Portier, M.M., Dunia, I., Paulin, D., Pournin, S., Babinet, C. Cell (1994) [Pubmed]
  37. Localization of newly synthesized vimentin subunits reveals a novel mechanism of intermediate filament assembly. Ngai, J., Coleman, T.R., Lazarides, E. Cell (1990) [Pubmed]
  38. Resistance to parvovirus B19 infection due to lack of virus receptor (erythrocyte P antigen). Brown, K.E., Hibbs, J.R., Gallinella, G., Anderson, S.M., Lehman, E.D., McCarthy, P., Young, N.S. N. Engl. J. Med. (1994) [Pubmed]
  39. High-affinity uptake of serotonin into immunocytochemically identified astrocytes. Kimelberg, H.K., Katz, D.M. Science (1985) [Pubmed]
  40. The iron transport protein NRAMP2 is an integral membrane glycoprotein that colocalizes with transferrin in recycling endosomes. Gruenheid, S., Canonne-Hergaux, F., Gauthier, S., Hackam, D.J., Grinstein, S., Gros, P. J. Exp. Med. (1999) [Pubmed]
WikiGenes - Universities