Clonal heterogeneity of synovial fluid T lymphocytes from patients with rheumatoid arthritis.
Although substantial evidence suggests that synovial T lymphocytes are critical in the pathogenesis of rheumatoid arthritis ( RA), little is known regarding their antigenic specificities, antigen receptor gene rearrangements, and mechanisms of activation. To assess the extent of expansion of specific clones among RA synovial fluid T cells, Southern blot analyses of T-cell receptor (TCR) gene rearrangements were performed on 40 RA synovial fluid T-cell clones, as well as on both fresh and polyclonally activated T cells from RA synovial fluid, RA peripheral blood, and normal peripheral blood. Two of the clones had identical TCR rearrangement patterns, but the remainder were unique. The nonclonal RA T-cell samples showed the same pattern of TCR beta-chain rearrangement that was observed among normal peripheral blood T cells, indicating no dominant clonal T-cell population in these samples. It was noted that with sufficient exposure of autoradiograms of the Southern blots, discrete TCR gene rearrangements, representing in some cases common D beta J beta (D, diversity; J, joining) rearrangements, were evident in T cells from peripheral blood of normal individuals and patients with RA, as well as T cells from RA synovial fluid. Taken together, the findings indicate that only a minor degree of oligoclonality can be demonstrated among T lymphocytes from RA synovial fluid.[1]References
- Clonal heterogeneity of synovial fluid T lymphocytes from patients with rheumatoid arthritis. Duby, A.D., Sinclair, A.K., Osborne-Lawrence, S.L., Zeldes, W., Kan, L., Fox, D.A. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
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