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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Citrate synthase: an immunochemical investigation of interspecies diversity.

Rabbit antibodies have been raised to pig heart citrate synthase. Using purified IgG, competitive enzyme-linked immunoassays and assays of citrate synthase activity indicate the presence of antibodies to a number of antigenic sites on the enzyme, only some of which are essential for catalytic activity. From a comparison of citrate synthases from prokaryotic and eukaryotic organisms, the degree of interaction between antibody and enzyme was in the order: pig heart greater than pigeon breast greater than Bacillus megaterium greater than Escherichia coli. These findings are discussed in terms of the known interspecies diversity of the enzyme.[1]


  1. Citrate synthase: an immunochemical investigation of interspecies diversity. Pullen, A.M., Budgen, N., Danson, M.J., Hough, D.W. FEBS Lett. (1985) [Pubmed]
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