Disease relevance of Bacillus megaterium

• Complexes of DNA with alpha/beta-type SASP from Bacillus cereus, Bacillus megaterium, or Clostridium bifermentans spores also gave spore photoproduct upon UV irradiation [1].
• Cloned DNA from Bacillus megaterium can complement mutants in the Escherichia coli proton-translocating ATPase [2].
• The amino acid composition was determined and compared with those of the glucose dehydrogenases from the archaebacterium Sulfolobus solfataricus and the eubacteria Bacillus subtilis and Bacillus megaterium [3].
• The results show that the activities of the majority of plant antimicrobials were considerably greater against the gram-positive bacteria Staphylococcus aureus and Bacillus megaterium and that disabling of the MDRs in gram-negative species leads to a striking increase in antimicrobial activity [4].
• The wall-covered bacteria Micrococcus lysodeikticus, Bacillus megaterium, and Proteus mirabilis incorporated exogenous cholesterol into their cytoplasmic membrane in quantities resembling those incorporated by sterol-nonrequiring mycoplasmas [5].

High impact information on Bacillus megaterium

• Bacillus megaterium resistance to cloxacillin accompanied by a compensatory change in penicillin binding proteins [6].
• Oxidation of elemental selenium to selenite by Bacillus megaterium [7].
• The energy requirements for the rapid transmembrane movement of phosphatidylethanolamine in membranes of Bacillus megaterium KM have been investigated by means of pulse label experiments [8].
• Membrane lateral phase separations and chlortetracycline transport by Bacillus megaterium [9].
• Protoplasts of two doubly auxotrophis strains of Bacillus megaterium were fused by nascent calcium phosphate or by polyethylene glycol treatment, and then allowed to revert to bacillary form on selection media [10].

Chemical compound and disease context of Bacillus megaterium

• The transbilayer distribution of phospholipids in Bacillus megaterium is asymmetrical, with twice as much phosphatidylethanolamine internally as externally (Rothaman, J. E. & Kennedy, E. P. (1977) J. Mol. Biol. 110,603-618) [11].
• Stability-increasing mutants of glucose dehydrogenase from Bacillus megaterium IWG3 [12].
• A unique cytochrome P-450-dependent fatty acid monooxygenase from Bacillus megaterium ATCC 14581 is strongly induced by phenobarbital (Narhi, L. O., and Fulco, A. J. (1982) J. Biol. Chem. 257, 2147-2150) and many other barbiturates (Kim, B.-H., and Fulco, A. J. (1983) Biochem. Biophys. Res. Commun. 116, 843-850) [13].
• Cell-free extracts from sonically disrupted Bacillus megaterium ATCC 13368 hydroxylated a variety of 3-oxo-delta4-steroids in position 15beta in the presence of NADPH and O2 [14].
• A protein factor, called PG-I, can be solubilized from toluene-treated Bacillus megaterium cells by LiCl extraction [15].

Biological context of Bacillus megaterium

• A glucose dehydrogenase gene was isolated from Bacillus megaterium IWG3, and its nucleotide sequence was identified [12].
• Mutants of Bacillus megaterium displaying malate-stimulated ATP synthesis resistant to uncouplers of oxidative phosphorylation were isolated and partially characterized [16].
• Analyses of the 5' regulatory sequences of genes encoding barbiturate-inducible cytochromes P450BM-1 and P450BM-3 from Bacillus megaterium and of the 5' sequences of genes for barbiturate-inducible P450b and P450e of the rat revealed a string of 17 base pairs in each of the genes that shared a high degree of sequence identity [17].
• In our previous publication (Shaw, G.-C., and Fulco, A. J. (1992) J. Biol. Chem. 267, 5515-5526), we reported that Bm3R1, a protein encoded in an open reading frame just upstream from the cytochrome P450BM-3 gene, is a repressor critically involved in the barbiturate-inducible expression of this gene in Bacillus megaterium [18].
• Analysis of the replicon region and identification of an rRNA operon on pBM400 of Bacillus megaterium QM B1551 [19].

Anatomical context of Bacillus megaterium

• Reconstitution of cell wall synthesis in toluene- and LiCl-treated Bacillus megaterium cells by addition of a soluble protein extract [20].
• The physical and kinetic properties of CS-II were similar to those of citrate synthase enzymes from Bacillus megaterium and from eukaryotic cells but differed from those of citrate synthases from many gram-negative bacteria [21].

Gene context of Bacillus megaterium

• We isolated a novel protease that converts plasminogen to angiostatin-like fragments (BL-angiostatins) from a culture of Bacillus megaterium A9542 through a single-step chromatography on CM-cellulose [22].
• The construction of a three-dimensional molecular model of the fungal form of cytochrome P450 (CYP51) from Saccharomyces cerevisiae, based on homology with the haemoprotein domain of CYP102 from Bacillus megaterium (a unique bacterial P450 of known crystal structure) is described [23].
• Xylose uptake in Bacillus megaterium depends on expression of a putative H+/xylose symporter encoded by xylT, the last gene in the xyl operon [24].
• Nucleotide sequence of a Bacillus megaterium gene homologous to the dnaK gene of Escherichia coli [25].
• Amino acid sequence analysis of tryptic peptides revealed significant similarity to the NADPH binding region of plant and animal NADPH-cytochrome P450 reductases and Bacillus megaterium cytochrome P450:NADPH-cytochrome P450 reductase [26].

Analytical, diagnostic and therapeutic context of Bacillus megaterium

• We isolated two galactosamine derivatives from Bacillus megaterium sporulating cells by lectin affinity chromatography followed by DEAE-Sephadex A-25 chromatography [27].
• Membranes from dormant and heat-activated spores of Bacillus megaterium QM B1551 were isolated and purified by gentle lysis procedures followed by differential and sucrose density gradient centrifugations [28].
• Comparison of the siderophores present in culture supernatants of R. solanacearum, R. metallidurans, and Bacillus megaterium using chemical tests, a siderophore utilization bioassay, thin-layer chromatography, and mass spectroscopy indicated that R. solanacearum produces staphyloferrin B rather than schizokinen as was reported previously [29].
• Evidence for an essential histidine residue in glucose dehydrogenase from Bacillus megaterium and sequence analysis of the peptides labeled with bromoacetyl pyridine [30].
• Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization [31].

References