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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

O-specific polysaccharides of Hafnia alvei lipopolysaccharides isolated from two serologically related strains: ATCC 13337 and 1187. A serological and structural study using chemical methods, gas chromatography/ mass spectrometry and NMR spectroscopy at 500 MHz.

The O-specific polysaccharides of Hafnia alvei ATCC 13337 standard strain and 1187 strain have been isolated and characterized. By means of 1H-NMR spectroscopy, methylation analysis and periodate oxidation, the repeating unit of the polysaccharides could be allocated the respective structures. (formula; see text) where Acyl = D-3-hydroxybutyryl, and 3-O-acetylation was to about 66%. The structural similarity of the polysaccharides was confirmed in the serological study; their epitopes were determined and the importance of various structural elements for the serological specificity was discussed.[1]


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