Enzymes as reagents in peptide synthesis: enzyme-labile protection for carboxyl groups.
N-Benzyloxycarbonyl derivatives of isoglutamine and isoasparagine were coupled with arginine methyl ester through the action of dicyclohexylcarbodiimide. The resulting benzyloxycarbonyl derivatives of isoglutaminylarginine methyl ester and isoasparaginylarginine methyl ester were treated with trypsin for removal of the ester groups and then with porcine pancreatic carboxypeptidase B for liberation of arginine and the original benzyloxycarbonyl derivatives of isoglutamine and isoasparagine. This scheme represents a reversible masking of the side-chain carboxyl functions of aspartic and glutamic acid residues. Possible applications of esters and amides of arginine as reversible blocking groups in protein semi-synthesis are discussed along with prospects and strategies for developing related techniques of higher efficiency.[1]References
- Enzymes as reagents in peptide synthesis: enzyme-labile protection for carboxyl groups. Glass, J., Pelzig, M. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
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