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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Human-mouse hybrids carrying fragments of single human chromosomes selected by tumor growth.

Fusion of human EJ bladder carcinoma cells to mouse C127 cells, with direct selection for tumor growth, gave rise to hybrid cells in which the human chromosome complement had been reduced dramatically, while selectively retaining the activated HRAS1 at chromosome band 11p15. A single-component hybrid retaining only part of human chromosome 11 is described in detail. Our results suggest a novel and general approach for investigating the chromosomal basis of neoplastic change and for subchromosomal mapping of and enrichment cloning for the human genome.[1]

References

  1. Human-mouse hybrids carrying fragments of single human chromosomes selected by tumor growth. Porteous, D.J., Wilkinson, M.M., Fletcher, J.M., van Heyningen, V. Genomics (1989) [Pubmed]
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