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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Synaptic facilitation by 3-aminopyridine and its antagonism by verapamil and diltiazem.

The effects of 3-aminopyridine (3-AP) on synaptic transmission in bullfrog sympathetic ganglion were studied in normal Ringer's solution and during graded reductions in extracellular Ca++ by means of intra- and extracellular recording techniques. 3-AP caused a single orthodromic stimulus to generate a brief burst of repetitive postganglionic discharges (SBR). In the absence of 3-AP, synaptic transmission, measured as the amplitude of the postganglionic compound action potential, failed progressively as Ca++ was reduced from 1.8 to 0.47 mM. This Ca++ dependence curve of synaptic transmission was shifted to the left (lower Ca++) by 3-AP in dose-related fashion, with maximum shift (4- to 5-fold) at 1 mM 3-AP. The magnitude of the maximum shift produced by 3-AP was precisely the same as that produced by 3,4-diaminopyridine and 4-aminopyridine. Although 3-AP could prevent transmission failure at otherwise suboptimal Ca++ levels, its ability to generate SBR failed progressively as Ca++ was reduced from normal (1.8 mM) to 0.5 mM. Thus, there was a wide difference between the Ca++ dependence domains of synaptic transmission and of SBR in the presence of 3-AP. To confirm this difference in Ca++ dependence domains by a method other than reduction of [Ca++]0, we investigated the interactions between 3-AP and two Ca++ entry blockers, verapamil and diltiazem. 3-AP SBR was abolished by verapamil and by diltiazem at concentrations significantly below those required to block synaptic transmission in the presence of 3-AP. The results thus demonstrate a competitive interaction between aminopyridines and Ca++ entry blockers and further confirm the Ca++ dependent nature of the synaptic actions of aminopyridines.[1]

References

  1. Synaptic facilitation by 3-aminopyridine and its antagonism by verapamil and diltiazem. Riker, W.K., Matsumoto, M., Takashima, K. J. Pharmacol. Exp. Ther. (1985) [Pubmed]
 
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