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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

A single tRNA (guanine)-methyltransferase from Tetrahymena with both mono- and di-methylating activity.

A tRNA (guanine-2) methyltransferase has been purified to homogeneity from the protozoan Tetrahymena pyriformis. The enzyme methylates purified E. coli tRNAs which have a guanine residue at position 26 from the 5' end; it also methylates tRNA prepared from the m22G- yeast mutant trm 1. This methyltransferase is therefore equivalent to the guanine methyltransferase 2mGII found in mammalian extracts. The purified 2mGII from Tetrahymena is capable of forming both N2-methylguanine and N22-dimethylguanine on a single tRNA isoaccepting species; under conditions of limiting tRNA or long reaction times the predominant product is dimethylguanine. Analysis of the products formed under varying reaction conditions suggests that dimethylguanine formation is a two step process requiring dissociation of the enzyme-monomethylated tRNA intermediate.[1]


  1. A single tRNA (guanine)-methyltransferase from Tetrahymena with both mono- and di-methylating activity. Reinhart, M.P., Lewis, J.M., Leboy, P.S. Nucleic Acids Res. (1986) [Pubmed]
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