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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Use of a quality-control plasma sample to decrease interassay variation in radioimmunoassays of apolipoprotein A-I.

The apolipoprotein A-I (apo A-I) radioimmunoassay established in our laboratory involves use of purified apo A-I as the primary standard for quantifying apo A-I in plasma and a pooled plasma (quality-control sample) as a secondary standard to decrease interassay variation. The measured values for apo A-I increased over time as the labeled antigen degraded. We observed these results in two separate studies: (a) apo A-I in plasma from 16 subjects was measured 12 times during four months, and (b) apo A-I in a single pooled plasma was measured 48 times during 10 months. We show that use of a quality-control plasma sample as a secondary standard decreased interassay variation, which was, in part, ascribable to degradation of the labeled antigen.[1]

References

  1. Use of a quality-control plasma sample to decrease interassay variation in radioimmunoassays of apolipoprotein A-I. Au, Y.P., Hallaway, B.J., Kottke, B.A. Clin. Chem. (1986) [Pubmed]
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