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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Expression of the bovine hypothalamic hormone oxytocin precursor in Escherichia coli.

The bovine oxytocin precursor was expressed in Escherichia coli as a fusion protein by cloning the hormone encoding cDNA in frame behind the replicase gene of the RNA phage MS2. By step-wise extraction with different urea concentrations, the fusion protein was enriched in the 7 M urea fraction and further purified by Sephacryl S-300 chromatography. The oxytocin precursor was cleaved off the fusion protein by cyanogen bromide treatment, chromatographed on FPLC columns and identified by Western blot analysis, using antibodies raised against neurophysin.[1]

References

  1. Expression of the bovine hypothalamic hormone oxytocin precursor in Escherichia coli. Nörenberg, U., Brunath, A., Richter, D. Biochimie (1988) [Pubmed]
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