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Gene Review

OXT  -  oxytocin/neurophysin I prepropeptide

Bos taurus

Synonyms: OT-NPI, OTNPI
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Disease relevance of OXT

  • We present a Raman and surface-enhanced Raman scattering (SERS) study of the following proteins containing S-S group(s): alpha chymotrypsin (alpha-CHT), insulin, lysozyme, oxytocin (OXT), Streptomyces subtilisin inhibitor (SSI), and trypsin inhibitor (STI) [1].
  • Mechanisms underlying the pathogenicity of diabetes insipidus mutations were probed by studying their effects on the properties of bovine oxytocin-related neurophysin [2].
  • To address this issue, the disulfide-rich precursor of oxytocin-associated bovine neurophysin was expressed in Escherichia coli and folded in vitro to yield milligram quantities of purified protein; evidence of significant impediments to yield resulting from damage to Cys residues is presented [3].
  • Concentrations of 10(-10) mol/l to 10(-1) mol/l suppressed progesterone secretion in a log dose-related manner (r = 0.97) with evidence of toxicity (lower oxytocin concentrations and significantly reduced DNA compared with controls) [4].
  • Endometria from pregnant cows did not respond to oxytocin when perifused at 39 degrees C. However, PGF secretion rates from endometrium of pregnant cows increased (P less than 0.01) in response to oxytocin when perifused under heat stress conditions.(ABSTRACT TRUNCATED AT 250 WORDS)[5]

Psychiatry related information on OXT


High impact information on OXT


Chemical compound and disease context of OXT


Biological context of OXT

  • The extracted AVP and oxytocin had identical retention times to those of the synthetic peptides on high-performance liquid chromatography (HPLC) and were biologically active in assays for antidiuretic and milk-ejection activity (with potencies of 310 units/mg and 340 units/mg respectively) [17].
  • Scatchard plots showed that each ligand interacted with a single high-affinity, low-capacity binding site: oxytocin dissociation constant (Kd) 3.1 +/- 0.29 nmol/l, maximum binding capacity (Bmax) 89.6 +/- 18.4 fmol/mg protein (n = 3); AVP Kd 0.73 +/- 0.02 nmol/l, Bmax 26.5 +/- 8.3 fmol/mg protein (n = 3).(ABSTRACT TRUNCATED AT 250 WORDS)[17]
  • In the present study, the oestrogen-dependent up-regulation of the bovine oxytocin promoter is investigated in MDA-MB 231 cells [18].
  • Transcriptional activation of the oxytocin promoter by oestrogens uses a novel non-classical mechanism of oestrogen receptor action [18].
  • Oxytocin and vasopressin receptors in bovine endometrium and myometrium during the estrous cycle and early pregnancy [19].

Anatomical context of OXT


Associations of OXT with chemical compounds


Physical interactions of OXT


Enzymatic interactions of OXT

  • The oxytocin precursor was cleaved off the fusion protein by cyanogen bromide treatment, chromatographed on FPLC columns and identified by Western blot analysis, using antibodies raised against neurophysin [28].

Regulatory relationships of OXT


Other interactions of OXT


Analytical, diagnostic and therapeutic context of OXT

  • The specificity of the immunoprecipitation was demonstrated by competition with excess amounts of unlabeled neurophysin II or arginine vasopressin; little or no competition was observed with unlabeled neurophysin II or arginine vasopressin; little or no competition was observed with unlabeled neurophysin I or oxytocin [35].
  • Fractions of gel chromatography effluent and extract of SDS-PAGE gel slices were subjected to RIA for immunoreactive precursors of AVP, AVP-NP, OT, and OT-NP using specific antisera fro each molecule [36].
  • In the present experiments we studied basal, prostaglandin (PG) F2 alpha-stimulated and ascorbate-stimulated OXT release from individual bovine luteal cells utilizing the reverse hemolytic plaque assay (RHPA) [37].
  • Oxytocinyl-GKR was shown by analytical affinity chromatography to bind noncovalently to neurophysin with an affinity close to that of mature oxytocin [38].
  • When activin-A was withdrawn from the cell culture after 72 h and the incubation continued for a further 72 h, a recovery in OT was seen on day 4 and 5 after activin-A doses of 0.1-1 ng/ml, but not after higher doses (3 and 10 ng/ml) [39].


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  16. Atosiban, an oxytocin receptor blocking agent: pharmacokinetics and inhibition of milk ejection in dairy cows. Wellnitz, O., Bruckmaier, R.M., Albrecht, C., Blum, J.W. J. Dairy Res. (1999) [Pubmed]
  17. Arginine vasopressin and oxytocin in the bovine adrenal gland. Nussey, S.S., Prysor-Jones, R.A., Taylor, A., Ang, V.T., Jenkins, J.S. J. Endocrinol. (1987) [Pubmed]
  18. Transcriptional activation of the oxytocin promoter by oestrogens uses a novel non-classical mechanism of oestrogen receptor action. Koohi, M.K., Ivell, R., Walther, N. J. Neuroendocrinol. (2005) [Pubmed]
  19. Oxytocin and vasopressin receptors in bovine endometrium and myometrium during the estrous cycle and early pregnancy. Fuchs, A.R., Behrens, O., Helmer, H., Liu, C.H., Barros, C.M., Fields, M.J. Endocrinology (1990) [Pubmed]
  20. Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophyses by radioimmunoassay. Landgraf, R. Endokrinologie. (1981) [Pubmed]
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  22. Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA. Land, H., Grez, M., Ruppert, S., Schmale, H., Rehbein, M., Richter, D., Schütz, G. Nature (1983) [Pubmed]
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