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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Physical and genetic characterization of cloned enterobactin genomic sequences from Escherichia coli K-12.

We have cloned genes responsible for enterobactin synthesis ( entD) and transport (fepA,fes) from Escherichia coli K-12. Relevant recombinant plasmids enabled EntD- and transport-defective mutants to grow on iron-limiting medium. Subcloning and deletion analysis demonstrated that the gene order is entD- fepA-fes. Protein synthesis studies in minicells suggest that FepA is first translated as an Mr 84 000 precursor, which is subsequently cleaved to the active Mr 81 000 receptor; the fes gene product is an Mr 44 000 protein; no polypeptide has been identified as the entD gene product.[1]

References

  1. Physical and genetic characterization of cloned enterobactin genomic sequences from Escherichia coli K-12. Fleming, T.P., Nahlik, M.S., Neilands, J.B., McIntosh, M.A. Gene (1985) [Pubmed]
 
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