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Experimentally-induced irritant contact dermatitis. Determination of optimum irritant concentrations.

Patch testing with 7 irritants has been performed on a panel of 42 healthy volunteers, with the aim of determining concentrations which would induce mild to moderate reactions in at least 75% of individuals tested. The irritants studied and their optimum concentrations were as follows: benzalkonium chloride, 0.5%; sodium lauryl sulphate, 5%; croton oil, 0.8%; dithranol, 0.02%; nonanoic acid, 80%; propylene glycol, 100%; sodium hydroxide, 2%. Responder rates lower than 75% had to be accepted for benzalkonium chloride and sodium hydroxide in order to prevent overly severe reactions, whilst propylene glycol proved to have only marginal irritant properties.[1]


  1. Experimentally-induced irritant contact dermatitis. Determination of optimum irritant concentrations. Willis, C.M., Stephens, J.M., Wilkinson, J.D. Contact Derm. (1988) [Pubmed]
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