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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization.

A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2----q11.2), although a secondary site of hybridization at q24----25 was also observed.[1]

References

  1. Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization. Liou, G.I., Fong, S.L., Gosden, J., van Tuinen, P., Ledbetter, D.H., Christie, S., Rout, D., Bhattacharya, S., Cook, R.G., Li, Y. Somat. Cell Mol. Genet. (1987) [Pubmed]
 
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