Spectrin oxidation correlates with membrane vesiculation in stored RBCs.
An increase in spectrin oxidation in a variety of erythrocytes displaying a tendency to vesiculate has been previously described. To explore this relationship in more detail, we have studied blood stored in citrate-phosphate-dextrose-adenine under blood bank conditions because, in this system, vesiculation occurs slowly. Vesiculation was quantitated by measuring acetylcholinesterase release, and the extent of spectrin oxidation was detected by using thiol-disulfide exchange chromatography. A strong correlation (r = .92) was found between the extent of spectrin oxidation and vesiculation when blood from five donors was analyzed at weekly intervals during storage. This strongly suggests that spectrin oxidation plays a role in the formation of spectrin-free vesicles, thereby limiting the shelf life of stored blood.[1]References
- Spectrin oxidation correlates with membrane vesiculation in stored RBCs. Wagner, G.M., Chiu, D.T., Qju, J.H., Heath, R.H., Lubin, B.H. Blood (1987) [Pubmed]
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