In vitro synthesis and processing of a bean pathogenesis-related (PR4) protein.
When bean plants (Phaseolus vulgaris var. Saxa) are treated with mercuric chloride or infected with alfalfa mosaic virus, they produce pathogenesis-related (PR) proteins. We report here that functional mRNA encoding bean PR4 protein is only present when synthesis of this protein has been induced. Treatment with mercuric chloride results in a rapid induction of functional bean PR4 mRNA (within 2-3 h), whereas in virus-infected plants this mRNA can only be detected the second day following the infection. Bean PR4 protein is synthesized in vitro, using this mRNA in a rabbit reticulocyte lysate system, as a precursor of 35 kDa. This precursor can be processed into a polypeptide having the same molecular mass (33.5 kDa) as the in vivo PR4 protein by the addition to the cell-free translation system of canine pancreatic microsomal membranes.[1]References
- In vitro synthesis and processing of a bean pathogenesis-related (PR4) protein. De Tapia, M., Dietrich, A., Burkard, G. Eur. J. Biochem. (1987) [Pubmed]
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