Disease relevance of Alfalfa Mosaic Virus

• This system was used to determine the concentrations of eIF-4C required for the half-maximal rate of translation of satellite tobacco necrosis virus RNA, alfalfa mosaic virus RNA 4, and barley alpha-amylase mRNA [1].
• Internal initiation was also studied with the RNA-dependent RNA polymerase of brome mosaic virus (BMV) and alfalfa mosaic virus (AlMV) [2].
• The 5' proximal ORF (ORF-1) is 882 nucleotides, encoding a gene product which shares homology with putative cell-to-cell movement proteins of related viruses, including tobacco streak virus (TSV) and alfalfa mosaic virus (AIMV) [3].
• A chimeric peptide containing antigenic determinants from rabies virus glycoprotein (G protein) (amino acids 253-275) and nucleoprotein (N protein) (amino acids 404-418) was PCR-amplified and cloned as a translational fusion product with the alfalfa mosaic virus (AlMV) coat protein (CP) [4].

High impact information on Alfalfa Mosaic Virus

• We used the untranslated leader from the coat protein mRNA of alfalfa mosaic virus (AMV RNA 4), a well-translated, highly competitive message, to replace the leader sequence of barley alpha-amylase (B alpha A) and human interleukin 1 beta (IL-1 beta) cDNAs [5].
• Initiation of polypeptide synthesis with various NH2-blocked aminoacyl-tRNAs under the direction of alfalfa mosaic virus RNA 4 [6].
• We report that the competitive translational activity of alfalfa mosaic virus coat protein mRNA (CP RNA), a nonadenylated mRNA, is determined in part by the 3' untranslated region (UTR) [7].
• Hydroxyl radical footprinting data show that five sites in the 3' UTR of alfalfa mosaic virus RNA 4 are protected by coat protein, and four of the five protected regions contain AUGC or UUGC [8].
• This polymerase activity was stimulated by oligonucleotides such as (A)nG, cap analogs such as m7GpppAm, and natural mRNAs such as alfalfa mosaic virus RNA 4 [9].

Chemical compound and disease context of Alfalfa Mosaic Virus

• Inhibition of alfalfa mosaic virus RNA and protein synthesis by actinomycin D and cycloheximide [10].
• The three-dimensional structure of the 3' terminus of alfalfa mosaic virus RNA in complex with an amino-terminal coat protein peptide revealed an unusual RNA fold with inter-AUGC basepairing stabilized by key arginine residues (Guogas, et al., 2004) [11].
• The reduced and carboxymethylated coat protein of alfalfa mosaic virus (AMV 425) was fragmented by means of cyanogen-bromide cleavage [12].
• When bean plants (Phaseolus vulgaris var. Saxa) are treated with mercuric chloride or infected with alfalfa mosaic virus, they produce pathogenesis-related (PR) proteins [13].
• Glutaraldehyde-fixation was shown to stabilize the structural integrity of alfalfa mosaic virus (AMV) particles as well as to increase their immunogenicity and antigenic reactivity [14].

Biological context of Alfalfa Mosaic Virus

• Tobacco plants transformed with the P1 and P2 replicase genes of alfalfa mosaic virus (AIMV) have been shown to produce functional replicase proteins, permitting their infection with AIMV inocula lacking the genome segments encoding P1 and P2, respectively [15].

Anatomical context of Alfalfa Mosaic Virus

• Actinomycin D, added early after inoculation, reduces the production of infectious alfalfa mosaic virus in cowpea protoplasts by 90% [10].

Gene context of Alfalfa Mosaic Virus

• Replicase-mediated resistance to alfalfa mosaic virus [15].
• RNA 3 of Alfalfa mosaic virus (AMV) encodes the movement protein (MP) and coat protein (CP) [16].
• The movement protein (MP) and coat protein (CP) encoded by Alfalfa mosaic virus (AMV) RNA 3 are both required for virus transport [17].
• Computer-assisted comparisons indicate an ancestral relationship between the 3a proteins of CMV, BMV, and alfalfa mosaic virus (AMV) and between the coat proteins of CMV and BMV [18].
• For expression of both UL54 and UL44, a truncated form of the alfalfa mosaic virus (AMV) RNA4 5'-UTR was found to be superior to the full-length AMV 5'-UTR or the original HCMV 5'-UTRs of different lengths [19].

Analytical, diagnostic and therapeutic context of Alfalfa Mosaic Virus

• Some observations on the binding properties of alfalfa mosaic virus to polystyrene and its significance to indirect ELISA [20].

References