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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Pectate lyase gene regulatory mutants of Erwinia chrysanthemi.

The pelB gene, which encodes one of the five pectate lyase isoenzymes of Erwinia chrysanthemi 3937, was mutagenized with a mini-Mu transposable element that can form gene fusions to the neomycin phosphotransferase-encoding region. Secondary mutants resistant to kanamycin in the absence of polygalacturonate, an inducer of wild-type pectate lyase activities, were selected. Such mutants produced other pectate lyase isoenzymes in the absence of the inducer.[1]


  1. Pectate lyase gene regulatory mutants of Erwinia chrysanthemi. Diolez, A., Richaud, F., Coleno, A. J. Bacteriol. (1986) [Pubmed]
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