The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Evidence for multiple condensing enzymes in rat hepatic microsomes catalyzing the condensation of saturated, monounsaturated, and polyunsaturated acyl coenzyme A.

The condensation of palmitoyl-CoA with malonyl-CoA by rat hepatic microsomes was competitively inhibited by myristoyl-CoA, whereas it was noncompetitively inhibited by palmitoleoyl and gamma-linolenoyl-CoA. Furthermore, the condensation of palmitoleoyl-CoA with malonyl-CoA was also noncompetitively inhibited by gamma-linolenoyl-CoA. Replacement of normal diet by a fat-free high carbohydrate diet resulted in 8-, 2.5-, and 2.3-fold increases in the condensation rates of both palmitoyl- and myristoyl-CoA, palmitoleoyl-CoA, and gamma-linolenoyl-CoA, respectively. On the other hand, administration of di-(2-ethylhexyl)phthalate (DEHP) resulted in a 2-fold stimulation of the condensation activities with myristoyl- and palmitoyl-CoA, while those with palmitoleoyl- and gamma-linolenoyl-CoA decreased to about 83 and 63%, respectively. Similar results following dietary changes or DEHP administration were obtained for total elongation activities. Finally condensation activities of 16:0, 16:1, and gamma-18:3 CoA were differently affected by the proteolytic enzyme, chymotrypsin. The competitive substrate studies, those of dietary and DEHP administration, and the differential action of chymotrypsin strongly suggest the existence of at least three discrete condensing enzymes catalyzing the condensation of saturated, monounsaturated, and polyunsaturated acyl-CoAs. These studies also indicate that the condensation reaction is the regulating and rate-limiting step of the fatty acid chain elongation system.[1]

References

 
WikiGenes - Universities