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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

B16 tumor cells contain a warfarin sensitive vitamin K1 2,3 epoxide reductase.

Using an adapted assay that requires an enzyme aliquot that forms only 5 pmoles vitamin K, we were able to demonstrate vitamin K1 2,3 epoxide reductase activity in cultured B16 mouse melanoma cells. The enzyme uses dithiothreitol, but not NADH as a reducing cofactor and is sensitive to inhibition by warfarin (2% residual activity at 10 micrograms/ml warfarin). Incubation of B16 cells in culture with 30 micrograms/ml warfarin leads to an 45% residual reductase as compared to normally cultured B16 cells. Combined with the reported presence of vitamin K dependent carboxylase in B16 cells and the cytotoxicity of warfarin towards B16 cells this suggests an active vitamin K cycle in these melanoma cells that may be essential for survival.[1]

References

  1. B16 tumor cells contain a warfarin sensitive vitamin K1 2,3 epoxide reductase. Uitendaal, M.P., Thijssen, H.H., Drittij-Reijnders, M.J., Hoeijmakers, M.J. Biochem. Biophys. Res. Commun. (1986) [Pubmed]
 
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