A secondary attachment site for bacteriophage lambda in trpC of E. coli.
We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the primary lambda attachment site: GCTTTTTTATACTAA. This 6 nucleotide sequence is also present in the intact trpC genome at the attachment site, as shown by analysis of trpC mRNA spanning this region.[1]References
- A secondary attachment site for bacteriophage lambda in trpC of E. coli. Christie, G.E., Platt, T. Cell (1979) [Pubmed]
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