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Isolation of the epidermal growth factor from the shrew submaxillary gland.

The epidermal growth factor ( EGF) from the submaxillary glands of the Chinese Shrew (Suncus murinus) is purified to apparent homogeneity by using a sequence of four chromatographic steps, viz. gel filtration on Sephacryl S-200, affinity chromatography on immobilized Ni, hydrophobic interaction on phenyl-Sepharose CL-4B and reverse-phase HPLC. An 800-fold increase in specific activity and an overall recovery of 46% were achieved. The most effective step in its purification is the successful use of immobilized metal ion affinity chromatography (IMAC). This method was very selective, reproducible and requires a minimum of sample pre-treatment prior to chromatography.[1]

References

  1. Isolation of the epidermal growth factor from the shrew submaxillary gland. Yip, T.T., Tam, Y.Y., Kong, Y.C., Belew, M.C., Porath, J. FEBS Lett. (1985) [Pubmed]
 
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