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Rode, W. et al.

Methionine synthase assay based on coupling with thymidylate synthase reaction.

Methionine synthase reaction may be coupled with thymidylate synthase-catalysed tritium release from [5-3H]dUMP via non-enzymatic reaction of formaldehyde with tetrahydrofolate. A convenient and sensitive assay of methionine synthase activity, based on this principle, is described.[1]

References

Methionine synthase assay based on coupling with thymidylate synthase reaction. Rode, W., Balińska, M. Acta Biochim. Pol. (1985) [Pubmed]

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