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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Differential fluorescent staining of human chromosomes with daunomycin and adriamycin--the d-bands.

Human chromosome preparations were treated with a group of anthracycline antibiotics. Well-defined, orange-red fluorescent bands were observed on chromosomes after the slide was stained with daunomycin and adriamycin but not with nogalamycin. The characteristic differential bands appeared to be similar to the banding patterns obtained by the quinacrine techniques. Interaction of these antibiotics with DNA could provide information on the general mechanism of fluorescent banding. Further, these bands (D-bands) appeared to be more stable than the Q-bands and may have some usefulness for routine clinical cytogenetic analysis.[1]


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