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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter.

We have determined the complete DNA sequence of a segment derived from Drosophila melanogaster that complements a yeast adenine-8 ( ade8) mutation. This sequence, in conjunction with transcriptional analysis, predicts that a 206 amino acid sequence accounts for complementation. We have ligated a 1.5 kb promoter segment of the yeast alcohol dehydrogenase I (ADHI) gene just upstream of the open reading frame. This construction results in ADE8+ function that responds to glucose and ethanol in a manner similar to that for the chromosomal ADHI gene, as determined by a simple quantitative color assay for ADE8+ function.[1]

References

  1. Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter. Henikoff, S., Furlong, C.E. Nucleic Acids Res. (1983) [Pubmed]
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