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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Differential localization of two myosins within nematode thick filaments.

The body wall muscle cells of the nematode, Caenorhabditis elegans, contain two unique types of myosin heavy chain, A and B. We have utilized an immunochemical approach to define the structural location of these two myosins within body wall muscle thick filaments. By immunofluorescence microscopy, myosin B antibodies label the thick filament-containing A-bands of body wall muscle with the exception of a thin gap at the center of each A-band, and myosin A antibodies react to form a medial fluorescent stripe within each A-band. The complexes of these monoclonal antibodies with isolated thick filaments were negatively stained and studied by electron microscopy. The myosin B antibody reacts with the polar regions of all filaments but does not react with a central 0.9 micron zone. The myosin A antibody reacts with a central 1.8 micron zone in all filaments but does not react with the polar regions.[1]

References

  1. Differential localization of two myosins within nematode thick filaments. Miller, D.M., Ortiz, I., Berliner, G.C., Epstein, H.F. Cell (1983) [Pubmed]
 
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