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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Disproportionate synthesis of Semiliki Forest virus 42S and 26S RNAs in polyamine-depleted baby hamster kidney cells.

The role of polyamines in viral RNA synthesis has been studied using Semliki Forest virus-infected, polyamine-depleted baby hamster kidney cells as a model system. The synthesis of viral 42S RNA, which corresponds to the viral genome, was markedly inhibited, while the synthesis of viral 26S RNA, which acts as a messenger for viral structural proteins, was reduced much less or not at all. The decreased total viral RNA synthesis and the ratio of 42S to 26S RNA were rapidly returned to normal by adding spermidine to the culture medium. From these results it can be hypothesized that polyamines have a special role in the synthesis of viral RNA, possibly affecting the conformation of the RNA template.[1]


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