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Gene Review

Ovgs1  -  miscRNA

Onyong-nyong virus

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Disease relevance of Ovgs1

  • The mRNA coding for the structural proteins of Semliki Forest virus, the 26S RNA, was injected into Xenopus oocytes [1].
  • One protein which comigrated with viral capsid protein was labeled under the direction of 26S RNA, and only one labeled peptide was detected after digestion with trypsin [2].
  • Most of the oligonucleotides were identical to those found in the mRNA (26S RNA) that codes for the virion structural proteins [3].
  • The 26S RNA-defective mutants of Sindbis virus were from two different complementation groups, A and G, indicating that functions of two viral nonstructural proteins ("A" and "G") are required in the regulation of the synthesis of 26S RNA [4].
  • The 26S RNA encodes a 1,324-amino-acid polyprotein exhibiting typical alphavirus structural protein organization [5].

High impact information on Ovgs1


Biological context of Ovgs1

  • The synthesis of viral 42S RNA, which corresponds to the viral genome, was markedly inhibited, while the synthesis of viral 26S RNA, which acts as a messenger for viral structural proteins, was reduced much less or not at all [9].
  • Studies using formyl--e135S]methionyl-tRNAf as precursor, and using diphtheria toxin and NAD to inhibit elongation, showed that 26S RNA contains only a single initiation site at, or near, the 5' end of the core protein cistron [10].

Anatomical context of Ovgs1

  • The SFV 42S RNA and the intracellular 26S RNA have been translated in a prokaryotic cell-free system, the E. coli S30 [11].
  • These structures, however, fulfilled the criteria of initiation complexes suggesting that there were more than one ribosome attached to each 42S RNA under conditions where 26S RNA bound only one [12].

Associations of Ovgs1 with chemical compounds

  • The decreased total viral RNA synthesis and the ratio of 42S to 26S RNA were rapidly returned to normal by adding spermidine to the culture medium [9].
  • After analysis on sucrose gradients the polysomal structures, containing prelabelled RNA and nascent peptide chains labelled with [35S]methionine, had sedimentation values from 100 to 200S in the case of 26S RNA and from 150 to over 250S with 42S RNA [12].


  1. Envelope proteins of Semliki Forest virus synthesized in Xenopus oocytes are transported to the cell surface. Huth, A., Rapoport, T.A., Kääriäinen, L. EMBO J. (1984) [Pubmed]
  2. Initiation of translation directed by 42S and 26S RNAs from Semliki Forest virus in vitro. Glanville, N., Ranki, M., Morser, J., Kääriäinen, L., Smith, A.E. Proc. Natl. Acad. Sci. U.S.A. (1976) [Pubmed]
  3. Defective interfering passages of Sindbis virus: nature of the defective virion RNA. Kennedy, S.I., Bruton, C.J., Weiss, B., Schlesinger, S. J. Virol. (1976) [Pubmed]
  4. Functional defects of RNA-negative temperature-sensitive mutants of Sindbis and Semliki Forest viruses. Keränen, S., Kääriäinen, L. J. Virol. (1979) [Pubmed]
  5. Rainbow trout sleeping disease virus is an atypical alphavirus. Villoing, S., Béarzotti, M., Chilmonczyk, S., Castric, J., Brémont, M. J. Virol. (2000) [Pubmed]
  6. 5'-Terminal nucleotide sequence of Semliki forest virus 18S defective interfering RNA is heterogeneous and different from the genomic 42S RNA. Pettersson, R.F. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  7. A procedure to verify an amino acid sequence which has been derived from a nucleotide sequence: application to the 26S RNA of Semliki Forest virus. Garoff, H., Riedel, H., Lehrach, H. Nucleic Acids Res. (1982) [Pubmed]
  8. Messenger ribonucleoprotein complexes containing in vitro-synthesized 26S and 42S Semliki Forest virus RNA. Michel, M.R. Arch. Virol. (1979) [Pubmed]
  9. Disproportionate synthesis of Semiliki Forest virus 42S and 26S RNAs in polyamine-depleted baby hamster kidney cells. Tuomi, K. Med. Biol. (1983) [Pubmed]
  10. Translation of the genes for the structural proteins of alphaviruses. Clegg, J.C., Kennedy, S.I. Med. Biol. (1975) [Pubmed]
  11. Translation of Semliki forest virus 42S and 26S RNAs in a cell-free system derived from Escherichia coli. Uomala, P., Glanville, N., Morser, J., Kääriäinen, L. Med. Biol. (1975) [Pubmed]
  12. Polysomes and initiation complexes in vitro induced by Semliki Forest virus 42S and 26S RNA. Ranki, M., Morser, J., Glanville, N. Med. Biol. (1975) [Pubmed]
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